Intermediate
Part:BBa_K2172000:Design
Designed by: Bowen Xiao Group: iGEM16_CIEI-BJ (2016-10-14)
GST-Thrombin-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 673
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
Design Notes
To insert the part into vectors, such as the ones provided by the iGEM competition, extra sticky ends must be added. The sequences of the sticky ends are added to the primers of the parts when performing PCR.
Source
The GST label comes from the vector pGEX-KG in E. coli.