Intermediate

Part:BBa_K2172000:Design

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


GST-Thrombin-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

To insert the part into vectors, such as the ones provided by the iGEM competition, extra sticky ends must be added. The sequences of the sticky ends are added to the primers of the parts when performing PCR.


Source

The GST label comes from the vector pGEX-KG in E. coli.

References